RNA-SEQ Overview

Whole rice grain of 4 pigmented (Bali, PH9, MRM16, MRQ100) and two white (MR297, MRQ76) rice varieties were obtained from MARDI rice germplasm. The dehusked rice grains were subjected for total RNA extraction using MLT method (Mornkham et al., 2013). High quality purified and DNAse treated total RNA was used for cDNA library construction and mRNA-seq next-generation sequencing. Rice transcriptome data from each rice varieties were mapped to Oryza sativa japonica cv. Nipponbarre reference genome using TopHat2 and Bowtie2. Transcript assembly was performed using Cufflink. Gene annotation was carried out based on rice gene annotation from RAPDB database. SNPs calling was carried out using GATK while SnpEff was used to annotate the SNPs. Genes Co-expression network analysis was performed using R scripts and Cytoscape.

Feature Summary